SF-1 Antibody / Steroidogenic Factor 1 / NR5A1 [clone NR5A1/3420] (V9468)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SF-1 antibody (clone NR5A1/3420). These results demonstrate the foremost specificity of the NR5A1/3420 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SF-1 Antibody Testis IHC. Immunohistochemistry analysis of FFPE human testis tissue stained with SF-1 Antibody demonstrates strong nuclear HRP-DAB brown staining within seminiferous tubule-associated cellular populations, consistent with expression of steroidogenic factor 1 / NR5A1 in gonadal and endocrine-associated transcriptionally active compartments. This steroidogenic factor 1 antibody highlights nuclear localization patterns associated with reproductive tissue differentiation, steroidogenic signaling, and endocrine lineage-associated transcriptional regulation pathways. Inset: PBS was used in place of primary antibody as a negative control to assess non-specific secondary antibody binding. Antibody concentration: 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.