Serum Response Factor Antibody / SRF [clone PCRP-SRF-1F7] (V9737)

IHC staining of FFPE human colon tissue with Serum Response Factor antibody (clone PCRP-SF7-1F1) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells using Serum Response Factor antibody (green, clone PCRP-SRF-1F7) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Serum Response Factor antibody (blue, clone PCRP-SRF-1F7) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free Serum Response Factor antibody (clone PCRP-SRF-1F7) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Serum Response Factor antibody (clone PCRP-SRF-1F7). These results demonstrate the foremost specificity of the PCRP-SRF-1F7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.