SERPINB5 Antibody / MASPIN [clone SERPINB5/4972] (V9413)

Immunohistochemistry analysis of SERPINB5 antibody in human skin. FFPE human skin tissue shows strong cytoplasmic and nuclear HRP-DAB brown staining in stratified squamous epithelial cells, with prominent signal in suprabasal keratinocytes. The underlying dermis is largely negative. The negative control inset, using PBS in place of primary antibody, shows no specific staining, confirming assay specificity. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9, 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human skin tissue with SERPINB5 antibody (clone SERPINB5/4972). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human PC3 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.

Western blot testing of human SW732 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERPINB5 antibody (clone SERPINB5/4972). These results demonstrate the foremost specificity of the SERPINB5/4972 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.