SERPINB5 Antibody / MASPIN [clone SERPINB5/4971] (V4580)

Immunohistochemistry analysis of SERPINB5 / MASPIN antibody in human skin. FFPE human skin tissue was stained with SERPINB5 / MASPIN antibody (clone SERPINB5/4971). HRP-DAB brown chromogenic signal is observed predominantly within the epidermal layer, showing strong cytoplasmic staining in keratinocytes with occasional nuclear positivity. The staining pattern highlights epithelial cells along the basal and suprabasal layers, consistent with the known epithelial expression of MASPIN. Dermal stromal components display minimal background staining. Nuclei are counterstained blue. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes followed by cooling prior to antibody incubation.

IHC staining of FFPE human lung tissue with SERPINB5 antibody (clone SERPINB5/4971). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free SERPINB5 antibody (clone SERPINB5/4971) as confirmation of integrity and purity.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using SERPINB5 antibody (clone SERPINB5/4971).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.