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Home >> Antibodies >> Serpin A8 Antibody / Angiotensinogen / AGT

Serpin A8 Antibody / Angiotensinogen / AGT (RQ8112)

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  Catalog No Formulation Size Price (USD)  
Image RQ8112 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectum adenocarcinoma tissue with Serpin A8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectum adenocarcinoma tissue with Serpin A8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Serpin A8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HCCT, 2) HCCP, 3) HepG2 and 4) HUH-7 cell lysate with Serpin A8 antibody. Predicted molecular weight ~52 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Serpin A8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serpin A8 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P01019
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Serpin A8 antibody is available for research use only.
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Description

AGT (ANGIOTENSINOGEN), also called SERPINA8, is an alpha-2-globulin that is produced constitutively and released into the circulation mainly by the liver. ATG is a member of the serpin family, although it is not known to inhibit other enzymes, unlike most serpins. Angiotensinogen is also known as renin substrate. The AGT gene is mapped on 1q42.2. And the human angiotensinogen gene contains 5 exons. The expression of AGT and enzymes required for its conversion to angiotensin II in human adipose tissue. Mutations in this AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Application Notes

Optimal dilution of the Serpin A8 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids D273-R458) was used as the immunogen for the Serpin A8 antibody.

Storage

After reconstitution, the Serpin A8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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