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Home >> Antibodies >> SERBP1 Antibody / PAI-RBP1

SERBP1 Antibody / PAI-RBP1 [clone PARB1-1] (V7625)

  Catalog No Formulation Size Price (USD)  
Image V7625-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V7625-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V7625SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V7625IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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IHC testing of FFPE human urothelial carcinoma with SERBP1 antibody (clone PARB1-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PARB1-1
Purity Protein G affinity chromatography
UniProt Q8NC51
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC Only Format : incubate for 30 min at RT (1)
Limitations This SERBP1 antibody is available for research use only.
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Description

May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay. Seems to play a role in PML-nuclear bodies formation. [UniProt]

For highly specific detection of SERBP1-associated RNA regulatory signaling pathways, see our SERBP1 Antibody / mRNA Binding Protein Antibody page featuring clone SERBP1/3491 with WB, IHC, and HuProt(TM) microarray specificity validation data.

Application Notes

The stated application concentrations are suggested starting points. Titration of the SERBP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A recombinant human partial protein corresponding to amino acids 3-139 was used as the immunogen for the SERBP1 antibody.

Storage

Store the SERBP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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