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Home >> Antibodies >> SEMA3B Antibody / Semaphorin 3B

SEMA3B Antibody / Semaphorin 3B [clone 9C4F7] (RQ6745)

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  Catalog No Formulation Size Price (USD)  
Image RQ6745 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with SEMA3B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with SEMA3B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human U-87 MG, 4) human K562, 5) rat kidney, 6) rat NRK, 7) mouse kidney and 8) mouse ANA-1 cell lysate with SEMA3B antibody. Predicted molecular weight ~83 kDa.
Flow cytometry testing of human HepG2 cells with SEMA3B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA3B antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 9C4F7
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q13214
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This SEMA3B antibody is available for research use only.
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  • Applications : WB, IHC-P, IF, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

Semaphorin-3B is a protein that in humans is encoded by the SEMA3B gene. It is mapped to 3p21.31. The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis.

Application Notes

Optimal dilution of the SEMA3B antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Y55-R624) was used as the immunogen for the SEMA3B antibody.

Storage

After reconstitution, the SEMA3B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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