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Home >> Antibodies >> Recombinant Secretory Component Glycoprotein Antibody / ECM1

Recombinant Secretory Component Glycoprotein Antibody / ECM1 [clone SRGP85] (V7065)

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  Catalog No Formulation Size Price (USD)  
Image V7065-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7065-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7065SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7065IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human colon carcinoma and Secretory Component Glycoprotein antibody (clone SRGP85). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma and Secretory Component Glycoprotein antibody (clone SRGP85). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name SRGP85
Purity Protein G affinity chromatography
UniProt Q16610
Localization Cell surface and cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Secretory Component Glycoprotein antibody is available for research use only.
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Description

Extracellular matrix protein 1 (ECM1), also called Secretory component p85, a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. [RefSeq]

Application Notes

Optimal dilution of the Secretory Component Glycoprotein antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the Secretory Component Glycoprotein antibody.

Storage

Store the Secretory Component Glycoprotein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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