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Home >> Antibodies >> SDHB Antibody / Succinate dehydrogenase B

SDHB Antibody / Succinate dehydrogenase B [clone SDHB/3745] (V4696)

  Catalog No Formulation Size Price (USD)  
Image V4696-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4696-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4696SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human liver tissue with SDHB antibody (clone SDHB/3745). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SDHB antibody (clone SDHB/3745) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name SDHB/3745
Purity Protein A/G affinity
UniProt P21912
Localization Cytoplasm (Mitochondria)
Applications Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This SDHB antibody is available for research use only.
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Description

Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.

Application Notes

Optimal dilution of the SDHB antibody should be determined by the researcher.

Immunogen

A recombinant human SDHB protein fragment (within amino acids 165-273) was used as the immunogen for the SDHB antibody.

Storage

Aliquot the SDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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