- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
SDHB Antibody / Complex II Monoclonal Antibody [clone SDHB/2382] (V8073)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V8073-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
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V8073-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
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V8073SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b, kappa |
| Clone Name | SDHB/2382 |
| Purity | Protein G affinity chromatography |
| UniProt | P21912 |
| Applications | Western Blot : 2-4ug/ml |
| Limitations | This SDHB Antibody / Complex II Monoclonal Antibody is available for research use only. |
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SDHB Antibody / Complex II Monoclonal Antibody detects succinate dehydrogenase complex iron sulfur subunit B, a nuclear-encoded component of mitochondrial respiratory Complex II. SDHB functions together with SDHA, SDHC and SDHD to form succinate dehydrogenase, an enzyme complex located in the inner mitochondrial membrane. Succinate dehydrogenase is unusual among respiratory chain complexes because it participates directly in both the tricarboxylic acid (TCA) cycle and mitochondrial electron transport, linking intermediary metabolism with oxidative phosphorylation.
During the TCA cycle, succinate dehydrogenase catalyzes the oxidation of succinate to fumarate. Electrons generated during this reaction are initially accepted by flavin adenine dinucleotide associated with SDHA and subsequently transferred through a series of iron-sulfur clusters contained within SDHB. Electron transfer then proceeds toward ubiquinone through the membrane-associated SDHC and SDHD components. SDHB therefore occupies a central position in the electron-transfer activity of mitochondrial Complex II.
Proper SDHB expression and Complex II assembly are important for mitochondrial respiration and cellular metabolic homeostasis. Loss or dysfunction of an SDH component can reduce succinate dehydrogenase activity and cause intracellular accumulation of succinate. Elevated succinate can inhibit alpha-ketoglutarate-dependent enzymes and alter oxygen-sensing and epigenetic regulatory mechanisms. These effects connect mitochondrial metabolic dysfunction with broader changes in cell signaling and gene regulation.
SDHB is particularly important in cancer research because pathogenic alterations of the SDHB gene are associated with hereditary paraganglioma and pheochromocytoma syndromes and have also been investigated in other SDH-deficient tumors. Succinate accumulation resulting from impaired SDH activity can contribute to tumor-associated metabolic reprogramming, leading to the classification of succinate as an oncometabolite. Evaluation of SDHB protein expression is consequently useful in research examining mitochondrial dysfunction, tumor metabolism and the molecular consequences of succinate dehydrogenase deficiency.
Clone SDHB/2382 is a mouse monoclonal antibody developed for recognition of SDHB. Protein microarray validation provides an additional approach for assessing antibody target recognition across a large collection of human proteins, complementing conventional experimental validation such as Western blot analysis. The defined monoclonal nature of SDHB/2382 also provides consistent recognition of its target epitope for studies of SDHB protein expression.
NSJ Bioreagents offers clone SDHB/2382 for research into mitochondrial Complex II and succinate dehydrogenase biology. This SDHB Antibody can support studies of mitochondrial respiration, TCA cycle metabolism, oxidative phosphorylation, SDH-deficient tumors and metabolic mechanisms associated with altered Complex II function.
Researchers studying succinate dehydrogenase expression and mitochondrial Complex II function can explore our SDHB Antibody page for additional reagents targeting the Complex II iron-sulfur subunit.
Optimal dilution of the SDHB Antibody / Complex II Monoclonal Antibody should be determined by the researcher.
A recombinant human partial protein (amino acids 165-273) were used as the immunogen for this SDHB antibody.
Store the SDHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
SDHB antibody, Complex II Monoclonal antibody, Succinate Dehydrogenase B antibody, Succinate Dehydrogenase Subunit B antibody, Succinate Dehydrogenase Iron-Sulfur Subunit antibody, Complex II Iron-Sulfur Subunit antibody, SDH2 antibody
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