SCO1 Antibody | RQ7435 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ7435	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	429
Formulation

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Immunofluorescent staining of FFPE human intestine cancer tissue with SCO1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human prostate cancer tissue with SCO1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

IHC staining of FFPE human lung cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human thyroid cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cervical cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate cancer tissue with SCO1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of human 1) Daudi, 2) 293T and 3) A549 cell lystate with SCO1 antibody. Predicted molecular weight ~34 kDa, commonly observed at 30-34 kDa.

Flow cytometry testing of human U-87 MG cells with SCO1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SCO1 antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	O75880
Localization	Cytoplasmic (mitochondria)
Applications	Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow cytometry : 1-3ug/million cells Immunofluorescence : 5ug/ml Direct ELISA : 0.1-0.5ug/ml
Limitations	This SCO1 antibody is available for research use only.
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Description

Protein SCO1 homolog, mitochondrial, also known as SCO1, cytochrome c oxidase assembly protein, is a protein that in humans is encoded by the SCO1 gene. Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene.

Application Notes

Optimal dilution of the SCO1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R21-S301) was used as the immunogen for the SCO1 antibody.

Storage

After reconstitution, the SCO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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SCO1 Antibody (RQ7435)

Description

Application Notes

Immunogen

Storage

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Area of Research

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Protocols

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