SCN4A Antibody / Sodium channel protein type 4 subunit alpha Antibody (FY13517)

SCN4A Antibody by IF in human U2OS cells. Immunofluorescence staining of U2OS cells using Sodium channel protein type 4 subunit alpha Antibody shows green immunoreactivity outlining portions of the cytoplasm and cell periphery, with blue DAPI nuclear counterstaining. The cells underwent enzymatic antigen retrieval for 15 minutes, followed by blocking with 10% goat serum. Primary antibody was applied at 5 ug/mL overnight at 4oC, and fluorescence was detected using a green fluorophore-conjugated anti-rabbit secondary antibody at 1:500. The signal is distinguishable from the nuclear counterstain and illustrates SCN4A-associated labeling in fixed cells.

SCN4A Antibody by WB in human and rodent samples. Western blot analysis using Sodium channel protein type 4 subunit alpha Antibody shows high-molecular-weight immunoreactivity near 250-300 kDa in eight samples. Lanes 1-4 contain human K562, Jurkat, THP-1 and U2OS cell lysates, respectively; lanes 5-6 contain rat small intestine and heart; lanes 7-8 contain mouse small intestine and heart. Thirty ug of reduced protein was loaded per lane on an 8% gel. The primary antibody was used at 0.5 ug/mL overnight at 4oC, followed by HRP-based detection. Band intensity varies across samples, with a particularly strong signal in K562 and U2OS lysates.

SCN4A Antibody by FACS in U2OS cells. Flow cytometry of fixed U2OS cells using Sodium channel protein type 4 subunit alpha Antibody shows a blue fluorescence histogram shifted to the right of the green isotype control and red unstained blank. Cells were fixed with 4% paraformaldehyde, blocked with normal goat serum, and incubated with 1 ug primary antibody per 1 x 10^6 cells. A fluorescent anti-rabbit secondary antibody was used for detection. The separated populations demonstrate antibody-associated staining in fixed U2OS cells; the assay does not establish cell-surface labeling in live cells.

SCN4A Antibody by FACS in THP-1 cells. Flow cytometry of fixed THP-1 cells using Sodium channel protein type 4 subunit alpha Antibody demonstrates a blue antibody-stained population shifted rightward relative to the green rabbit IgG isotype control and red unstained blank. Cells were fixed with 4% paraformaldehyde and blocked with normal goat serum before incubation with 1 ug primary antibody per 1 x 10^6 cells. Fluorescence was detected with an anti-rabbit secondary antibody. The histogram documents SCN4A-associated staining in fixed THP-1 cells without establishing reactivity on the surface of viable cells.