- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
SCLT1 Antibody / Sodium channel and clathrin linker 1 (FY12490)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12490 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
SCLT1 antibody detects Sodium channel and clathrin linker 1, a centrosomal and ciliary base protein essential for ciliogenesis and centrosome organization. SCLT1 participates in the distal appendage complex of centrioles, which anchors the mother centriole to the plasma membrane during primary cilia formation. The SCLT1 antibody is widely used in cell biology and developmental studies to investigate ciliary assembly, centriole architecture, and cilia-dependent signaling pathways.
SCLT1 is encoded by the SCLT1 gene located on human chromosome 4q28.1. The protein is approximately 68 kilodaltons and is conserved among vertebrates. It contains coiled-coil regions that mediate binding to other distal appendage proteins, including CEP164, FBF1, and CEP83. SCLT1 provides structural linkage between microtubule-based centrioles and the membrane docking machinery required for ciliogenesis initiation. Knockout or mutation of SCLT1 disrupts distal appendage integrity and prevents cilia formation.
The SCLT1 antibody detects the protein by western blot as a 68 kilodalton band and reveals punctate centrosomal staining under immunofluorescence microscopy. Localization studies show that SCLT1 is present at the distal end of the mother centriole and co-localizes with ciliary initiation markers. Functional analyses demonstrate that SCLT1 depletion inhibits ciliary vesicle docking, leading to defective Hedgehog signaling and altered tissue morphogenesis. Mutations in SCLT1 have been linked to ciliopathies such as Senior-Løken syndrome and orofaciodigital spectrum disorders, highlighting its importance in organ development.
Beyond its role in ciliogenesis, SCLT1 may interact with ion channels and clathrin adaptor proteins, potentially linking ciliary membrane organization with endocytic trafficking. It has also been implicated in cell cycle control through centrosome duplication regulation. NSJ Bioreagents provides a validated SCLT1 antibody optimized for western blot, immunofluorescence, and confocal imaging applications, enabling detailed investigation of centrosomal structure, cilia formation, and associated signaling processes.
Optimal dilution of the SCLT1 antibody should be determined by the researcher.
E.coli-derived human SCLT1 recombinant protein (Position: F19-I688) was used as the immunogen for the SCLT1 antibody.
After reconstitution, the SCLT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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