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Home >> Antibodies >> SARS-CoV-2 Nucleocapsid Protein Antibody (2019-nCov)

SARS-CoV-2 Nucleocapsid Protein Antibody (2019-nCov) [clone RM420] (R20435)

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  Catalog No Formulation Size Price (USD)  
Image R20435-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 419
Microvalidated Recrabbitmono
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Binding curve of anti-NP (2019-nCov) RM420 monoclonal antibody and recombinant 2019-nCov Nucleocapsid Protein. ELISA plate was coated with 50ng recombinant 2019-nCov Nucleocapsid Protein at concentration of 1ug/ml. A 2-fold serial dilution from 125 ng/ml was performed using this recombinant SARS-CoV-2 Nucleocapsid Protein antibody. For detection, AP-conjugated goat anti-rabbit IgG secondary antibody was used.
Binding curve of anti-NP (2019-nCov) RM420 monoclonal antibody and recombinant 2019-nCov Nucleocapsid Protein. ELISA plate was coated with 50ng recombinant 2019-nCov Nucleocapsid Protein at concentration of 1ug/ml. A 2-fold serial dilution from 125 ng/ml was performed using this recombinant SARS-CoV-2 Nucleocapsid Protein antibody. For detection, AP-conjugated goat anti-rabbit IgG secondary antibody was used.
Availability 1-3 business days
Species Reactivity SARS-CoV
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM420
Purity Protein A purified from animal origin-free supernatant
Applications Use at an assay dependent concentration :
Limitations This recombinant SARS-CoV-2 Nucleocapsid Protein antibody is available for research use only.
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Description

This antibody reacts to the Nucleocapsid Protein of SARS-CoV-2 (2019-nCov). No cross-reactivity to common SARS coronaviruses observed. It can be used as the capture antibody in a Sandwich ELISA assay with clone RMH03 monoclonal antibody.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant SARS-CoV-2 Nucleocapsid Protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

The binding activity of N protein with anti-N protein antibody was verified with the sensitivity of 0.1-0.3 ng/ml.

Immunogen

A recombinant 2019-nCov Nucleocapsid Protein expressed in E.coli was used as the immunogen for the recombinant SARS-CoV-2 Nucleocapsid Protein antibody.

Storage

Store the recombinant SARS-CoV-2 Nucleocapsid Protein antibody at -20oC.

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