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Home >> Antibodies >> Sarcomeric Actinin Alpha 2 Antibody / ACTN2

Sarcomeric Actinin Alpha 2 Antibody / ACTN2 [clone ACTN2/8184R] (V4814)

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  Catalog No Formulation Size Price (USD)  
Image V4814-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4814-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4814SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human skeletal muscle tissue with ACTN2 antibody (clone ACTN2/8184R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human skeletal muscle tissue with ACTN2 antibody (clone ACTN2/8184R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Sarcomeric Actinin Alpha 2 antibody (clone ACTN2/8184R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ACTN2/8184R
Purity Protein A/G affinity
UniProt P35609
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Sarcomeric Actinin Alpha 2 antibody is available for research use only.
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Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Sarcomeric Actinin Alpha 2 antibody should be determined by the researcher.

Immunogen

A portin of amino acids 557-692 from the human protein was used as the immunogen for the Sarcomeric Actinin Alpha 2 antibody.

Storage

Aliquot the Sarcomeric Actinin Alpha 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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