SAMHD1 Antibody / SAMHD1 Polyclonal Antibody (RQ5761)

SAMHD1 Antibody WB Analysis of HeLa, HepG2, K562, Raji and NIH 3T3 Cells. Western blot analysis of human HeLa (lane 1), HepG2 (lane 2), K562 (lane 3), Raji (lane 4), and mouse NIH 3T3 (lane 5) cell lysates using SAMHD1 Polyclonal Antibody. A prominent band is detected near 72 kDa in all five samples, consistent with the predicted molecular weight of SAMHD1. The results demonstrate detection of SAMHD1-sized proteins in human and mouse cell lysates.

SAMHD1 Antibody IHC Staining of Human Lung Cancer Tissue. Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung cancer tissue using SAMHD1 Polyclonal Antibody. Brown DAB staining is observed in numerous cells, with prominent nuclear staining in portions of the tissue. Hematoxylin provides blue nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

SAMHD1 Antibody IHC Staining of Human Rectal Cancer with Lymphoid Infiltration. Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal cancer tissue using SAMHD1 Polyclonal Antibody. Brown DAB staining is observed in scattered cells surrounding glandular structures, while numerous smaller cells in the densely cellular regions show predominantly blue hematoxylin counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

SAMHD1 Antibody IHC Staining of Glandular Structures in Human Rectal Cancer. Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectal cancer tissue using SAMHD1 Polyclonal Antibody. Brown DAB staining is visible in portions of the glandular epithelium and surrounding tissue, with variation in staining intensity across the examined field. Hematoxylin provides nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

SAMHD1 Antibody IHC Staining of Rat Intestinal Tissue. Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat intestinal tissue using SAMHD1 Polyclonal Antibody. Brown DAB staining is visible along portions of the intestinal glandular epithelium and in surrounding tissue regions, with hematoxylin nuclear counterstaining. Heat-induced epitope retrieval was performed using EDTA buffer (pH 8) for 20 minutes.

SAMHD1 Antibody IF Staining of Human A549 Cells. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human A549 cells using SAMHD1 Polyclonal Antibody. SAMHD1-associated fluorescence is shown in green, with nuclei counterstained using DAPI (blue). Green fluorescence is visible in a subset of cells, including regions overlapping the nuclear staining. Heat-induced epitope retrieval was performed using citrate buffer (pH 6) for 20 minutes.

SAMHD1 Antibody FACS Staining of Human U-2 OS Cells. Flow cytometry analysis of human U-2 OS cells using SAMHD1 Polyclonal Antibody at 1 ug per million cells. The antibody-stained population (blue) shows a rightward fluorescence shift relative to the isotype control (green) and unstained cells (red), demonstrating antibody-associated staining consistent with SAMHD1 detection. Cells were blocked with goat serum before staining.

SAMHD1 Antibody WB Validation in HEK293 Wild-Type and SAMHD1 Knockout Cells. Western blot analysis of human HEK293 wild-type (WT) and SAMHD1 knockout (KO) whole-cell lysates using SAMHD1 Polyclonal Antibody at 0.5 ug/ml. A prominent band near 70 kDa in WT cells is markedly reduced in the KO sample, supporting SAMHD1-dependent recognition. A faint residual band remains near the corresponding position in the KO lane. GAPDH serves as the loading control. Each lane contained 30 ug of total protein.