SAM68 Antibody | KHDRBS1 | RQ8189 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ8189	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

SAM68 Antibody IF Staining of Rat Brain Tissue. Immunofluorescence analysis of FFPE rat brain tissue using KHDRBS1 Polyclonal Antibody. Red immunofluorescence is observed in numerous cells throughout the tissue section, with prominent signal in rounded cellular profiles. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Mouse Brain Tissue. Immunofluorescence analysis of FFPE mouse brain tissue using KHDRBS1 Polyclonal Antibody. Red fluorescence is observed in numerous cells throughout the tissue section, with prominent signal in rounded cellular profiles. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Human Intestinal Cancer Tissue. Immunofluorescence analysis of FFPE human intestinal cancer tissue using KHDRBS1 Polyclonal Antibody. Red immunofluorescence is observed in tumor-associated epithelial structures, with substantial signal overlapping the blue DAPI nuclear counterstain. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Human A549 Cells. Immunofluorescence analysis of FFPE human A549 cells using KHDRBS1 Polyclonal Antibody. SAM68 immunoreactivity is shown in red, with prominent nuclear localization, while Beta Tubulin is visualized in green throughout the cytoplasmic microtubule network. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Papillary Thyroid Carcinoma Tissue. Immunohistochemical analysis of FFPE human papillary thyroid carcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is observed in tumor-associated epithelial cells, with staining involving numerous nuclei. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Ovarian Adenocarcinoma Tissue. Immunohistochemical analysis of FFPE human ovarian adenocarcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown staining is observed in scattered tumor cell nuclei, with variation in staining across the tissue section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Keratinizing Squamous Cell Carcinoma Tissue. Immunohistochemical analysis of FFPE human keratinizing squamous cell carcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is visible in scattered nuclei within the tumor tissue, with heterogeneous staining among cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Placental Tissue. Immunohistochemical analysis of FFPE human placenta using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is observed in cells associated with placental villous structures, including nuclear staining in portions of the tissue. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Spleen Tissue. Immunohistochemical analysis of FFPE human spleen tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is observed in a subset of cells distributed throughout the splenic tissue, with regional differences in staining. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Glioblastoma Tissue. Immunohistochemical analysis of FFPE human glioblastoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is visible in scattered tumor-associated cells, including nuclear staining, against a predominantly hematoxylin-counterstained background. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Mouse Brain Tissue. Immunohistochemical analysis of FFPE mouse brain tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is observed in numerous cells, with particularly prominent staining in a densely cellular region of the section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Rat Brain Tissue. Immunohistochemical analysis of FFPE rat brain tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is visible in scattered cells throughout the brain parenchyma, with variation in staining among individual cellular profiles. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody WB Analysis of Human HeLa, 293T, Raji and Daudi Cells, Rat Brain and PC-12 Cells, and Mouse Brain and NIH3T3 Cells. Western blot analysis of human HeLa (lane 1), 293T (lane 2), Raji (lane 3), and Daudi (lane 4) cell lysates; rat brain (lane 5) and PC-12 (lane 6) lysates; and mouse brain (lane 7) and NIH3T3 (lane 8) lysates using KHDRBS1 Polyclonal Antibody. An immunoreactive band is detected near 62-68 kDa across all eight samples. Although the calculated molecular weight of KHDRBS1 is approximately 48 kDa, SAM68 is commonly observed migrating near 68 kDa.

SAM68 Antibody FACS Analysis of Human Raji Cells. Flow cytometry analysis of fixed and permeabilized human Raji cells using KHDRBS1 Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. The blue histogram represents antibody-stained cells, the green histogram represents the isotype control, and the red histogram represents unstained cells. A pronounced rightward fluorescence shift is observed in the antibody-stained population relative to both controls.

SAM68 Antibody WB Analysis of Wild-Type and KHDRBS1 Knockout HeLa Cells. Western blot analysis of human HeLa wild-type (WT, lane 1) and KHDRBS1 knockout (KO, lane 2) whole-cell lysates using KHDRBS1 Polyclonal Antibody at 0.5 ug/ml. A prominent immunoreactive band near 68-70 kDa is detected in WT lysate and is absent from the KO sample, supporting SAM68-specific recognition. Although the calculated molecular weight of KHDRBS1 is approximately 48 kDa, SAM68 is commonly observed migrating near 68 kDa. GAPDH serves as the loading control.

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SAM68 Antibody IF Staining of Rat Brain Tissue. Immunofluorescence analysis of FFPE rat brain tissue using KHDRBS1 Polyclonal Antibody. Red immunofluorescence is observed in numerous cells throughout the tissue section, with prominent signal in rounded cellular profiles. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Mouse Brain Tissue. Immunofluorescence analysis of FFPE mouse brain tissue using KHDRBS1 Polyclonal Antibody. Red fluorescence is observed in numerous cells throughout the tissue section, with prominent signal in rounded cellular profiles. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Human Intestinal Cancer Tissue. Immunofluorescence analysis of FFPE human intestinal cancer tissue using KHDRBS1 Polyclonal Antibody. Red immunofluorescence is observed in tumor-associated epithelial structures, with substantial signal overlapping the blue DAPI nuclear counterstain. Heat-induced epitope retrieval was performed by steaming sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IF Staining of Human A549 Cells. Immunofluorescence analysis of FFPE human A549 cells using KHDRBS1 Polyclonal Antibody. SAM68 immunoreactivity is shown in red, with prominent nuclear localization, while Beta Tubulin is visualized in green throughout the cytoplasmic microtubule network. Heat-induced epitope retrieval was performed by steaming in pH 6 citrate buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Papillary Thyroid Carcinoma Tissue. Immunohistochemical analysis of FFPE human papillary thyroid carcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is observed in tumor-associated epithelial cells, with staining involving numerous nuclei. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Ovarian Adenocarcinoma Tissue. Immunohistochemical analysis of FFPE human ovarian adenocarcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown staining is observed in scattered tumor cell nuclei, with variation in staining across the tissue section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Keratinizing Squamous Cell Carcinoma Tissue. Immunohistochemical analysis of FFPE human keratinizing squamous cell carcinoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is visible in scattered nuclei within the tumor tissue, with heterogeneous staining among cells. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Placental Tissue. Immunohistochemical analysis of FFPE human placenta using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is observed in cells associated with placental villous structures, including nuclear staining in portions of the tissue. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Spleen Tissue. Immunohistochemical analysis of FFPE human spleen tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is observed in a subset of cells distributed throughout the splenic tissue, with regional differences in staining. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Human Glioblastoma Tissue. Immunohistochemical analysis of FFPE human glioblastoma tissue using KHDRBS1 Polyclonal Antibody. Brown immunoreactivity is visible in scattered tumor-associated cells, including nuclear staining, against a predominantly hematoxylin-counterstained background. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Mouse Brain Tissue. Immunohistochemical analysis of FFPE mouse brain tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is observed in numerous cells, with particularly prominent staining in a densely cellular region of the section. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody IHC Staining of Rat Brain Tissue. Immunohistochemical analysis of FFPE rat brain tissue using KHDRBS1 Polyclonal Antibody. Brown nuclear immunoreactivity is visible in scattered cells throughout the brain parenchyma, with variation in staining among individual cellular profiles. Heat-induced epitope retrieval was performed by boiling sections in pH 8 EDTA buffer for 20 minutes.

SAM68 Antibody WB Analysis of Human HeLa, 293T, Raji and Daudi Cells, Rat Brain and PC-12 Cells, and Mouse Brain and NIH3T3 Cells. Western blot analysis of human HeLa (lane 1), 293T (lane 2), Raji (lane 3), and Daudi (lane 4) cell lysates; rat brain (lane 5) and PC-12 (lane 6) lysates; and mouse brain (lane 7) and NIH3T3 (lane 8) lysates using KHDRBS1 Polyclonal Antibody. An immunoreactive band is detected near 62-68 kDa across all eight samples. Although the calculated molecular weight of KHDRBS1 is approximately 48 kDa, SAM68 is commonly observed migrating near 68 kDa.

SAM68 Antibody FACS Analysis of Human Raji Cells. Flow cytometry analysis of fixed and permeabilized human Raji cells using KHDRBS1 Polyclonal Antibody at 1 ug per million cells following blocking with goat serum. The blue histogram represents antibody-stained cells, the green histogram represents the isotype control, and the red histogram represents unstained cells. A pronounced rightward fluorescence shift is observed in the antibody-stained population relative to both controls.

SAM68 Antibody WB Analysis of Wild-Type and KHDRBS1 Knockout HeLa Cells. Western blot analysis of human HeLa wild-type (WT, lane 1) and KHDRBS1 knockout (KO, lane 2) whole-cell lysates using KHDRBS1 Polyclonal Antibody at 0.5 ug/ml. A prominent immunoreactive band near 68-70 kDa is detected in WT lysate and is absent from the KO sample, supporting SAM68-specific recognition. Although the calculated molecular weight of KHDRBS1 is approximately 48 kDa, SAM68 is commonly observed migrating near 68 kDa. GAPDH serves as the loading control.

Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q07666
Localization	Nuclear, cytoplasmic
Applications	Western blot: 0.5-1ug/ml Immunohistochemistry (FFPE): 2-5ug/ml Immunofluorescence: 5ug/ml Flow cytometry: 1-3ug/million cells Direct ELISA: 0.1-0.5ug/ml
Limitations	This SAM68 Antibody / KHDRBS1 Polyclonal Antibody is available for research use only.
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Description

SAM68 Antibody / KHDRBS1 Polyclonal Antibody targets Src-associated in mitosis 68 kDa protein (SAM68), an RNA-binding protein encoded by the KHDRBS1 gene. SAM68 belongs to the STAR (signal transduction and activation of RNA) family of proteins, which integrate extracellular signaling pathways with the regulation of RNA metabolism. Through its interactions with RNA and signaling proteins, SAM68 participates in alternative splicing, transcript processing, cell-cycle regulation, and cellular differentiation.

SAM68 contains a conserved GSG domain that includes a K homology (KH) RNA-binding domain and regions involved in protein interactions. The KH domain recognizes selected RNA sequences, allowing SAM68 to influence the processing of specific transcripts. Additional proline-rich regions mediate interactions with signaling proteins containing SH3 domains, while tyrosine residues provide regulatory sites for phosphorylation. These structural features connect RNA-binding activity with changes in cellular signaling.

One of the principal functions of SAM68 is the regulation of alternative pre-mRNA splicing. By interacting with selected RNA sequences and components of the splicing machinery, SAM68 influences exon inclusion or exclusion in transcripts associated with cell survival, proliferation, and differentiation. Its activity can be modified by phosphorylation, methylation, and other post-translational modifications. These regulatory mechanisms influence RNA binding, protein interactions, and subcellular distribution, allowing SAM68 to respond to changing cellular conditions.

SAM68 was originally characterized through its association with Src-family tyrosine kinases during mitosis. Subsequent research established its broader role in linking signal transduction to RNA processing. SAM68 participates in pathways involving cellular proliferation, apoptosis, and responses to extracellular signals. Its functions also extend to neuronal development and RNA regulation in differentiated tissues, where coordinated alternative splicing contributes to specialized cellular activities.

Altered SAM68 expression and activity have been investigated in several cancers, including breast, prostate, and colorectal malignancies. Depending on the cellular context, SAM68 can influence the processing of transcripts involved in tumor-associated signaling and cell survival. Its biological effects depend on the availability of interacting proteins, phosphorylation status, and the transcripts expressed within individual cells.

SAM68 Antibody supports research into the relationship between RNA-binding proteins and cellular signaling pathways. Characterizing KHDRBS1 expression and regulation contributes to understanding alternative splicing, RNA processing, and the mechanisms through which signaling events influence gene expression.

Explore additional antibodies targeting RNA-binding proteins, splicing regulators, and proteins involved in cellular signaling in our Cell Biology Antibodies collection.

Application Notes

Optimal dilution of the SAM68 Antibody / KHDRBS1 Polyclonal Antibodyy should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M21-E239) was used as the immunogen for the SAM68 antibody.

Storage

After reconstitution, the SAM68 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

KHDRBS1 antibody, Src-associated in mitosis 68 kDa protein antibody, Src-associated in mitosis 68 antibody, SAM68 RNA-binding protein antibody, KH domain-containing RNA-binding signal transduction-associated protein 1 antibody, KH domain-containing protein antibody, STAR family RNA-binding protein antibody, KHDRBS1 RNA-binding protein antibody, SAM68 splicing regulator antibody, SAM68 signal transduction protein antibody

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SAM68 Antibody / KHDRBS1 Polyclonal Antibody (RQ8189)

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