SAFB Antibody / Scaffold attachment factor B1 (R32563)

SAFB Antibody Human Colon Cancer IF. Immunofluorescence staining of FFPE human colon cancer tissue with SAFB Antibody demonstrated strong nuclear localization in malignant epithelial cells (red), while nuclei were counterstained with DAPI (blue). Heat-induced epitope retrieval was performed in pH8 EDTA buffer before incubation with the primary antibody and a Fluoro550-conjugated secondary antibody. The staining pattern is consistent with the function of SAFB as a Scaffold Attachment Factor involved in chromatin organization, transcriptional regulation and RNA processing. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunofluorescence studies of nuclear protein expression in cancer and gene regulation research.

SAFB Antibody Human Prostate Cancer IHC. Immunohistochemical staining of FFPE human prostate cancer tissue demonstrated moderate to strong SAFB expression with predominant nuclear staining in malignant glandular epithelial cells and weaker staining of the surrounding stroma. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The nuclear localization is consistent with the role of Scaffold Attachment Factor in chromatin organization, transcriptional regulation and RNA processing. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunohistochemical studies of nuclear protein expression in prostate cancer and gene regulation research.

SAFB Antibody Human Colon Cancer IHC. Immunohistochemical staining of FFPE human colon cancer tissue demonstrated moderate to strong SAFB expression with predominantly nuclear staining in neoplastic epithelial cells and weaker staining in the surrounding stroma. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The nuclear localization is consistent with the established role of SAFB as a Scaffold Attachment Factor involved in chromatin organization, transcriptional regulation and RNA processing. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunohistochemical studies of nuclear protein expression in cancer and gene regulation research.

SAFB Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil demonstrated widespread SAFB expression with predominant nuclear staining throughout the lymphoid follicles and interfollicular regions. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The nuclear staining pattern is consistent with the role of Scaffold Attachment Factor in chromatin organization, transcriptional regulation and RNA processing within proliferating immune cells. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunohistochemical studies of nuclear protein expression in lymphoid tissues and immune cell biology.

SAFB Antibody T47D Cell IF. Immunofluorescence staining of T47D cells with SAFB Antibody demonstrated predominant nuclear localization with a punctate staining pattern characteristic of nuclear speckles involved in RNA processing and transcriptional regulation. Cells underwent enzyme-mediated antigen retrieval before incubation with the primary antibody and a Fluoro488-conjugated secondary antibody. The observed staining is consistent with the function of SAFB as a Scaffold Attachment Factor that associates with chromatin and nuclear RNA-processing complexes. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunofluorescence studies of nuclear organization, gene regulation and RNA metabolism.

SAFB Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain demonstrated widespread SAFB expression with prominent nuclear staining in neurons and other brain cells, accompanied by weaker diffuse cytoplasmic staining. Heat-induced epitope retrieval was performed in pH8 EDTA buffer before staining with the primary antibody and HRP-DAB detection. The predominantly nuclear localization is consistent with the role of Scaffold Attachment Factor in chromatin organization, transcriptional regulation and RNA processing within the central nervous system. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for immunohistochemical studies of neuronal gene regulation and brain tissue biology.

SAFB Antibody Human Cancer Cell WB. Western blot analysis detected SAFB in human K562 (lane 1), Caco-2 (lane 2), PC-3 (lane 3) and MCF-7 (lane 4) cell lysates. A predominant immunoreactive band was observed at approximately 150 kDa, migrating higher than the predicted molecular weight of about 103 kDa, consistent with the reduced electrophoretic mobility reported for this multifunctional nuclear protein. SAFB is known to undergo extensive post-translational modification and participate in large nuclear protein complexes, which can influence its apparent migration. This SAFB Antibody, a Scaffold Attachment Factor Antibody, is suitable for detecting endogenous SAFB in studies of transcriptional regulation, chromatin organization and RNA processing.