S100B Antibody / Brain Injury Biomarker [clone S100B/4152] (V4847)

Immunohistochemistry analysis of S100B / Brain Injury Biomarker antibody (clone S100B/4152) in human brain tissue. FFPE human brain demonstrates strong cytoplasmic and nuclear HRP-DAB brown staining in astrocyte-like cells distributed throughout the parenchyma, consistent with S100B expression. Staining highlights cells with branching processes characteristic of astroglial morphology, while neuronal cell bodies show comparatively weaker signal. Nuclei are counterstained blue. The inset image represents a secondary antibody negative control in which PBS was used in place of the primary antibody and shows absence of specific staining. Heat induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free S100B/Brain Injury Biomarker antibody (clone S100B/4152) as confirmation of integrity and purity.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100B/Brain Injury Biomarker antibody (clone S100B/4152). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to be specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.