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Home >> Antibodies >> S100A9 Antibody

S100A9 Antibody (R31896)

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  Catalog No Formulation Size Price (USD)  
Image R31896 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human A431 and 2) MCF7 cell lysate with S100A9 antibody. Expected/observed molecular weight ~14 kDa.
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Western blot testing of 1) human A431 and 2) MCF7 cell lysate with S100A9 antibody. Expected/observed molecular weight ~14 kDa.
IHC testing of FFPE human appendicitis tissue with S100A9 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human tonsil with S100A9 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P06702
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Limitations This S100A9 antibody is available for research use only.
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Description

S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.

Application Notes

Optimal dilution of the S100A9 antibody should be determined by the researcher.

Immunogen

Amino acids 2-114 of human S100A9 were used as the immunogen for the S100A9 antibody.

Storage

After reconstitution, the S100A9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Calprotectin L1H subunit , MRP14, Calgranulin B

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