S100A16 Antibody / Epithelial Differentiation Marker [clone S100A16/7411] (V4719)

S100A16 Antibody Glandular Tumor IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human colon carcinoma tissue using mouse monoclonal S100A16 antibody, clone S100A16/7411. HRP-DAB brown staining highlights cytoplasmic and membranous labeling of tumor epithelial cells forming glandular structures, consistent with epithelial differentiation, while surrounding stromal cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody Intestinal Epithelium IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human duodenum tissue using mouse monoclonal S100A16 antibody, clone S100A16/7411. HRP-DAB brown staining highlights strong cytoplasmic and membranous labeling of enterocytes lining villous structures, consistent with epithelial differentiation, while lamina propria cells show minimal staining and nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody Epidermal Layer IHC. Immunohistochemistry of S100 calcium binding protein A16 / S100A16 in FFPE human skin tissue using mouse monoclonal S100A16 antibody, clone S100A16/7411. HRP-DAB brown staining highlights strong cytoplasmic and membranous labeling of keratinocytes across the epidermal layers, consistent with epithelial differentiation, while underlying dermal components show minimal staining and nuclei are counterstained blue. Inset: PBS was used in place of primary antibody as a negative control to confirm specificity of staining. Heat induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to staining.

S100A16 Antibody SK-BR-3 WB. Western blot analysis of S100 calcium binding protein A16 / S100A16 in human SK-BR-3 cell lysate using S100A16 antibody. A band is detected at approximately 10-12 kDa, consistent with the predicted molecular weight of S100A16, supporting detection of this small calcium-binding protein in epithelial-derived cells.

SDS-PAGE analysis of purified, BSA-free S100A16 antibody (clone S100A16/7411) as confirmation of integrity and purity.