S100A10 Antibody | RQ4239 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	RQ4239	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

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Immunofluorescent staining of FFPE human lung cancer tissue with S100A10 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.

Immunofluorescent staining of FFPE human U-2 OS cells with S100A10 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human breast cancer tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human intestinal diffuse large B-cell lymphoma tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung adenocarcinoma tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with S100A10 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human A431, 2) human PC-3, 3) human pancreas, 4) human Caco-2, 5) human plasma 6) monkey lung, 7) rat lung, 8) rat PC-12, 9) mouse lung and 10) mouse Hepa 1-6 cell lysate with S100A10 antibody at 0.5ug/ml. Predicted molecular weight ~11 kDa.

Western blot testing of 1) human placenta, 2) human A431, 3) human HaCaT, 4) human U-87 MG, 5) monkey lung, 6) rat PC-12 and 7) mouse NIH 3T3 cell lysate with S100A10 antibody at 0.5ug/ml. Predicted molecular weight ~11 kDa.

Flow cytometry testing of fixed and permeabilized human A431 cells with S100A10 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= S100A10 antibody.

Availability	1-3 business days
Species Reactivity	Human, Mouse, Rat, Monkey
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt	P60903
Localization	Cytoplasmic, membrane
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence/Immunocytochemistry : 2-4ug/ml Flow Cytometry : 1-3ug/10^6 cells Direct ELISA : 0.1-0.5ug/ml
Limitations	This S100A10 antibody is available for research use only.
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Description

S100 calcium-binding protein A10 (S100A10), also known as p11, is a protein that is encoded by the S100A10 gene in humans and the S100a10 gene in other species. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

Application Notes

Optimal dilution of the S100A10 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids Q4-K94 was used as the immunogen for the S100A10 antibody.

Storage

After reconstitution, the S100A10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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S100A10 Antibody (RQ4239)

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