• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> S100 Calcium-Binding Protein B Antibody / Pan-Tissue Validated Marker

S100 Calcium-Binding Protein B Antibody / Pan-Tissue Validated Marker [clone MSVA-490R] (V5988)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image V5988-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 559
Image
V5988-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 259
Microvalidated Recrabbitmono
Bulk quote request
Immunohistochemistry analysis of S100 calcium-binding protein B / S100B antibody (clone MSVA-490R) in human tissue microarrays. Recombinant rabbit monoclonal S100-B antibody was evaluated on FFPE normal and cancer tissue microarrays. In normal tissues, staining is predominantly cytoplasmic and nuclear in astrocytes of the central nervous system and in melanocytic and selected stromal cell populations, consistent with known S100B distribution. In cancer tissues, strong cytoplasmic and nuclear HRP-DAB brown staining is observed in melanoma and selected neural and soft tissue tumors, while many epithelial malignancies show absent or minimal signal. The observed immunoreactivity pattern across normal and tumor specimens aligns with established expression data reported in the Human Protein Atlas, supporting the antibody’s specificity and suitability for comparative tissue expression studies.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-490R
UniProt P04271
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This S100 Calcium-Binding Protein B antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

S100 Calcium-Binding Protein B antibody, also known as Pan-Tissue Validated Marker antibody, recognizes S100 calcium-binding protein B, a small EF-hand calcium sensor encoded by the human S100B gene located on chromosome 21q22.3. S100 calcium-binding protein B is a member of the S100 family of calcium-binding proteins that function as intracellular regulators of cytoskeletal dynamics, enzyme activity, and transcriptional control. The protein forms homodimers and undergoes conformational change upon calcium binding, enabling interaction with diverse signaling partners.

S100B is most prominently expressed in astrocytes within the central nervous system, where it contributes to calcium-dependent signaling and regulation of glial cell morphology. However, expression is not restricted to neural tissue. S100 calcium-binding protein B is also detected in melanocytes, Schwann cells, adipocytes, chondrocytes, and selected epithelial and stromal cell populations. Because of this lineage-specific but multi-tissue distribution, S100B has become an important marker in both neurobiology and oncology research.

In cancer biology, S100B expression is well documented in melanoma and certain glial tumors, where it is frequently used as a diagnostic marker. Broader tissue microarray analysis demonstrates that S100B expression patterns in normal and malignant tissues align with large-scale transcriptomic and proteomic datasets, including those reported in the Human Protein Atlas. This consistency supports its reliability as a research marker for lineage identification and tumor characterization.

As a Pan-Tissue Validated Marker antibody, this recombinant monoclonal clone supports immunohistochemical evaluation across a wide range of normal and cancer tissues. Its staining profile reflects established expression patterns of S100 calcium-binding protein B and enables comparative analysis of physiologic and pathologic expression. S100 Calcium-Binding Protein B antibody is suitable for research applications investigating neurobiology, tumor biology, lineage tracing, and tissue-specific protein expression patterns.

Application Notes

1. Optimal dilution of the S100 Calcium-Binding Protein B antibody should be determined by the researcher.

2. This S100 Calcium-Binding Protein B antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

Recombinant full-length human S100B protein was used as the immunogen for the S100 Calcium-Binding Protein B antibody.

Storage

S100 Calcium-Binding Protein B antibody with sodium azide store at 2 to 8oC; antibody without sodium azide store at -20 to -80oC.

Related Products

   1.   View all S100B/S100 calcium-binding protein B antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.