S100 beta Antibody / S100B [clone S100B/4149] (V9572)

Immunohistochemistry analysis of S100 beta / S100B antibody (clone S100B/4149) in human brain tissue. FFPE human brain demonstrates diffuse cytoplasmic and nuclear HRP-DAB brown staining in astrocyte-like cells distributed throughout the parenchyma, consistent with S100B expression. Staining highlights cells with stellate morphology and branching processes characteristic of astroglial cells, while neuronal cell bodies show comparatively weaker signal. Background staining is minimal under the conditions tested. Nuclei are counterstained blue. Heat induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free S100 beta/S100B antibody (clone S100B/4149) as confirmation of integrity and purity.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using S100 beta/S100B antibody (clone S100B/4149). These results demonstrate the foremost specificity of the S100B/4149 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.