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Home >> Antibodies >> RUNX1T1 Antibody

RUNX1T1 Antibody (RQ4332)

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  Catalog No Formulation Size Price (USD)  
Image RQ4332 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human placenta and 2) mouse testis lysate with RUNX1T1 antibody at 0.5ug/ml. Predicted molecular weight ~67 kDa.
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Western blot testing of 1) human placenta and 2) mouse testis lysate with RUNX1T1 antibody at 0.5ug/ml. Predicted molecular weight ~67 kDa.
IHC testing of FFPE human breast cancer tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse brain tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IF/ICC staining of FFPE human U-2 OS cells with RUNX1T1 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with RUNX1T1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUNX1T1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q06455
Localization Nucleus
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence/Immunocytochemistry : 2-4ug/ml
Flow cytometry : 1-3ug/ml
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This RUNX1T1 antibody is available for research use only.
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Description

Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the RUNX1T1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids T335-D510 was used as the immunogen for the RUNX1T1 antibody.

Storage

After reconstitution, the RUNX1T1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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