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Home >> Antibodies >> RUFY3 Antibody / RIPx

RUFY3 Antibody / RIPx (RQ8197)

  Catalog No Formulation Size Price (USD)  
Image RQ8197 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with RUFY3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUFY3 antibody.
Western blot testing of 1) rat brain and 2) mouse brain tissue lysate with RUFY3 antibody. Predicted molecular weight: 53-70 kDa (multiple isoforms).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7L099
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This RUFY3 antibody is available for research use only.
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Description

The RUFY3 gene, also called RUN and FYVE domain-containing protein 3 and Rap2-interacting protein x (RIPx), encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the RUFY3 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-H469) was used as the immunogen for the RUFY3 antibody.

Storage

After reconstitution, the RUFY3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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