RTN3 Antibody / Reticulon 3 Polyclonal Antibody (R31380)

RTN3 Antibody staining of human brain neurons by IHC. Immunohistochemistry of FFPE human brain sections using the RTN3 Antibody at 2 ug/ml. Strong brown staining is visible throughout the neuropil, with distinct cytoplasmic labeling of numerous neuronal cell bodies. Nuclei are counterstained blue and remain largely negative. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer before overnight primary antibody incubation at 4oC. The predominantly cytoplasmic staining pattern is consistent with the intracellular membrane-associated localization of Reticulon 3 and demonstrates detection with this Reticulon 3 Polyclonal Antibody.

RTN3 Antibody detection in human, rat and mouse samples by WB. Western blot testing with the RTN3 Antibody under reducing conditions. Lane 1: human 293T; lane 2: human HeLa; lane 3: human U87; lane 4: human U2OS; lane 5: rat brain; lane 6: rat C6; lane 7: mouse brain; lane 8: mouse Neuro-2a. A prominent RTN3-reactive band is detected at approximately 25 kDa across the tested samples. Although the longest RTN3 isoform has a calculated molecular weight of approximately 113 kDa, multiple shorter RTN3 isoforms are reported in the approximately 24-28 kDa range, consistent with the observed migration detected by this Reticulon 3 Polyclonal Antibody.