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Home >> Antibodies >> RTF1 Antibody / RNA polymerase-associated protein RTF1 homolog

RTF1 Antibody / RNA polymerase-associated protein RTF1 homolog (RQ7901)

  Catalog No Formulation Size Price (USD)  
Image RQ7901 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE mouse brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with RTF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human SW620, 3) human MCF7 and 4) monkey COS-7 cell lysate with RTF1 antibody. Predicted molecular weight ~80 kDa, commonly observed at 80-100 kDa.
Flow cytometry testing of human Daudi cells with RTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RTF1 antibody.
Availability 1-3 business days
Species Reactivity Human, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q92541
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunohistochemistry (FFPE) : 2-5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This RTF1 antibody is available for research use only.
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Description

Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) is a protein that in humans is encoded by the RTF1 gene. This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA.

Application Notes

Optimal dilution of the RTF1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids S77-K705) was used as the immunogen for the RTF1 antibody.

Storage

After reconstitution, the RTF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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