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Home >> Antibodies >> RPS17 Antibody / 40S ribosomal protein S17

RPS17 Antibody / 40S ribosomal protein S17 (RQ7890)

  Catalog No Formulation Size Price (USD)  
Image RQ7890 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human PC-3 cells with RPS17 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human Thp-1, 3) human 293T, 4) human placenta, 5) rat ovary, 6) rat RH35 and 7) mouse NIH 3T3 cell lysate with RPS17 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human A431 cells with RPS17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPS17 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P08708
Localization Cytoplasm, nucleus, nucleolus
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This RPS17 antibody is available for research use only.
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Description

40S ribosomal protein S17 is a protein that in humans is encoded by the RPS17 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Application Notes

Optimal dilution of the RPS17 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-V135) was used as the immunogen for the RPS17 antibody.

Storage

After reconstitution, the RPS17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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