RPS12 Antibody | 40S Ribosomal Protein S12 Antibody FY13479

	Catalog No	Formulation	Size	Price (USD)
	FY13479	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

RPS12 Antibody Human Thyroid Cancer IHC. Immunohistochemical analysis of FFPE human thyroid cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic thyroid epithelial cells, consistent with the expected cytoplasmic localization of the 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human thyroid cancer tissue.

RPS12 Antibody Human Liver Cancer IHC. Immunohistochemical analysis of FFPE human liver cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed throughout the hepatocellular tumor cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human liver cancer tissue.

RPS12 Antibody Human Bladder Cancer IHC. Immunohistochemical analysis of FFPE human bladder cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed within the neoplastic urothelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 as a component of actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human bladder cancer tissue.

RPS12 Antibody Human Clear Cell Renal Cell Carcinoma IHC. Immunohistochemical analysis of FFPE human clear cell renal cell carcinoma tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Diffuse cytoplasmic staining is observed throughout the clear cell renal carcinoma cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human clear cell renal cell carcinoma tissue.

RPS12 Antibody Human Rectal Cancer IHC. Immunohistochemical analysis of FFPE human rectal cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human rectal cancer tissue.

RPS12 Antibody Human Ovarian Cancer IHC. Immunohistochemical analysis of FFPE human ovarian cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic ovarian epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human ovarian cancer tissue.

RPS12 Antibody Human Colon Cancer IHC. Immunohistochemical analysis of FFPE human colon cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong diffuse cytoplasmic staining is observed throughout the neoplastic glandular epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human colon cancer tissue.

RPS12 Antibody Mouse Ovary IHC. Immunohistochemical analysis of FFPE mouse ovary tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed throughout the ovarian cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE mouse ovary tissue.

RPS12 Antibody Mouse Brain IHC. Immunohistochemical analysis of FFPE mouse brain tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Diffuse cytoplasmic staining is observed throughout neurons, consistent with the expected localization of 40S ribosomal protein S12 as a constituent of the cytosolic ribosome and actively translating protein synthesis machinery. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE mouse brain tissue.

RPS12 Antibody Rat Brain IHC. Immunohistochemical analysis of FFPE rat brain tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4Â°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed in neurons, including prominent labeling within the densely packed neuronal layer, consistent with the expected localization of 40S ribosomal protein S12 as a component of the cytosolic ribosome and actively translating protein synthesis machinery. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE rat brain tissue.

RPS12 Antibody Human, Rat and Mouse Cell WB. Western blot analysis of RPS12 Antibody using lysates from 1) human HeLa cells, 2) human HepG2 cells, 3) rat C6 cells, 4) mouse RAW264.7 cells, and 5) mouse NIH/3T3 cells. Samples (30 ug/lane) were separated on a 12% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and probed with RPS12 Antibody (0.5 ug/mL), followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is detected at approximately 15 kDa in all five cell lysates, consistent with the predicted molecular weight of RPS12. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 across human, rat, and mouse cell lines.

RPS12 Antibody MCF-7 Cell FACS. Flow cytometric analysis of fixed and permeabilized MCF-7 cells using RPS12 Antibody. Cells were fixed with 4% paraformaldehyde, permeabilized, blocked with normal goat serum, and incubated with RPS12 Antibody (1 ug/10^6 cells) for 30 minutes at 20Â°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used for detection. The blue histogram represents RPS12 staining, the green histogram is the rabbit IgG isotype control, and the red histogram is the unstained control. A distinct rightward shift of the RPS12-stained population relative to the controls demonstrates specific intracellular detection of endogenous RPS12. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, is suitable for flow cytometric analysis of intracellular RPS12 expression.

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RPS12 Antibody Human Thyroid Cancer IHC. Immunohistochemical analysis of FFPE human thyroid cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic thyroid epithelial cells, consistent with the expected cytoplasmic localization of the 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human thyroid cancer tissue.

RPS12 Antibody Human Liver Cancer IHC. Immunohistochemical analysis of FFPE human liver cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed throughout the hepatocellular tumor cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human liver cancer tissue.

RPS12 Antibody Human Bladder Cancer IHC. Immunohistochemical analysis of FFPE human bladder cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed within the neoplastic urothelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 as a component of actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human bladder cancer tissue.

RPS12 Antibody Human Clear Cell Renal Cell Carcinoma IHC. Immunohistochemical analysis of FFPE human clear cell renal cell carcinoma tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Diffuse cytoplasmic staining is observed throughout the clear cell renal carcinoma cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human clear cell renal cell carcinoma tissue.

RPS12 Antibody Human Rectal Cancer IHC. Immunohistochemical analysis of FFPE human rectal cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human rectal cancer tissue.

RPS12 Antibody Human Ovarian Cancer IHC. Immunohistochemical analysis of FFPE human ovarian cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate to strong diffuse cytoplasmic staining is observed throughout the neoplastic ovarian epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human ovarian cancer tissue.

RPS12 Antibody Human Colon Cancer IHC. Immunohistochemical analysis of FFPE human colon cancer tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong diffuse cytoplasmic staining is observed throughout the neoplastic glandular epithelial cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE human colon cancer tissue.

RPS12 Antibody Mouse Ovary IHC. Immunohistochemical analysis of FFPE mouse ovary tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed throughout the ovarian cells, consistent with the expected cytoplasmic localization of 40S ribosomal protein S12 within actively translating ribosomes. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE mouse ovary tissue.

RPS12 Antibody Mouse Brain IHC. Immunohistochemical analysis of FFPE mouse brain tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Diffuse cytoplasmic staining is observed throughout neurons, consistent with the expected localization of 40S ribosomal protein S12 as a constituent of the cytosolic ribosome and actively translating protein synthesis machinery. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE mouse brain tissue.

RPS12 Antibody Rat Brain IHC. Immunohistochemical analysis of FFPE rat brain tissue using RPS12 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody (1:100) overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate diffuse cytoplasmic staining is observed in neurons, including prominent labeling within the densely packed neuronal layer, consistent with the expected localization of 40S ribosomal protein S12 as a component of the cytosolic ribosome and actively translating protein synthesis machinery. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 in FFPE rat brain tissue.

RPS12 Antibody Human, Rat and Mouse Cell WB. Western blot analysis of RPS12 Antibody using lysates from 1) human HeLa cells, 2) human HepG2 cells, 3) rat C6 cells, 4) mouse RAW264.7 cells, and 5) mouse NIH/3T3 cells. Samples (30 ug/lane) were separated on a 12% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and probed with RPS12 Antibody (0.5 ug/mL), followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is detected at approximately 15 kDa in all five cell lysates, consistent with the predicted molecular weight of RPS12. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, specifically detects endogenous RPS12 across human, rat, and mouse cell lines.

RPS12 Antibody MCF-7 Cell FACS. Flow cytometric analysis of fixed and permeabilized MCF-7 cells using RPS12 Antibody. Cells were fixed with 4% paraformaldehyde, permeabilized, blocked with normal goat serum, and incubated with RPS12 Antibody (1 ug/10^6 cells) for 30 minutes at 20°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used for detection. The blue histogram represents RPS12 staining, the green histogram is the rabbit IgG isotype control, and the red histogram is the unstained control. A distinct rightward shift of the RPS12-stained population relative to the controls demonstrates specific intracellular detection of endogenous RPS12. These results demonstrate that RPS12 Antibody, a 40S Ribosomal Protein S12 Antibody, is suitable for flow cytometric analysis of intracellular RPS12 expression.

Species Reactivity	Human, Mouse, Rat
Format	Lyophilized
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P25398
Applications	Western Blot : 0.5-1ug/ml ELISA : 0.1-0.5ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells
Limitations	This RPS12 Antibody / 40S Ribosomal Protein S12 Antibody is available for research use only.
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Description

RPS12 Antibody recognizes 40S Ribosomal Protein S12 (RPS12), a highly conserved structural component of the small 40S ribosomal subunit that is essential for accurate messenger RNA translation and protein synthesis. As part of the cytosolic ribosome, RPS12 contributes to ribosome assembly, messenger RNA decoding, and maintenance of translational fidelity during peptide elongation. Because efficient protein synthesis is fundamental to virtually every cellular process, RPS12 is expressed in nearly all cell types and tissues. Consequently, RPS12 Antibody is widely used to investigate ribosome biology, translational regulation, cellular growth, and mechanisms controlling protein production.

RPS12 participates in the formation and stabilization of the ribosomal decoding center, where it helps ensure accurate codon recognition and transfer RNA selection during translation. Although traditionally viewed as a housekeeping protein, increasing evidence indicates that ribosomal proteins can influence selective messenger RNA translation, stress adaptation, cell cycle progression, and cellular differentiation. Alterations in ribosome composition or function may affect the translation of specific transcripts involved in proliferation, apoptosis, metabolism, and development. Accordingly, RPS12 Antibody has become an important research tool for studies of ribosome biogenesis, translational control, RNA biology, and cellular responses to physiological stress.

Disruption of ribosomal protein function has been linked to a growing group of disorders collectively known as ribosomopathies, while dysregulated protein synthesis is increasingly recognized as a hallmark of cancer and other proliferative diseases. Although pathogenic variants in RPS12 are uncommon compared with some other ribosomal proteins, altered ribosome function and translational regulation contribute broadly to tumor development, metabolic disorders, neurodegeneration, and responses to cellular stress. Researchers commonly employ RPS12 Antibody in Western blotting, immunohistochemistry, immunofluorescence, immunoprecipitation, and related protein expression studies to characterize ribosomal protein abundance, localization, and translational activity across normal and diseased tissues.

NSJ Bioreagents provides RPS12 Antibody products that undergo validation for research applications to support reliable detection of this essential ribosomal protein. Whether investigating ribosome assembly, messenger RNA translation, protein synthesis, cell growth, or translational regulation, a high-quality 40S Ribosomal Protein S12 Antibody enables accurate characterization of RPS12 expression and localization. As interest in translational control and ribosome biology continues to expand across biomedical research, RPS12 Antibody remains an indispensable tool for studying the molecular mechanisms that govern cytosolic protein synthesis.

Explore our Cell Biology Antibodies page to discover additional antibodies against ribosomal proteins, translation factors, RNA-binding proteins, and other regulators of protein synthesis that complement RPS12 Antibody research.

Application Notes

Optimal dilution of the RPS12 Antibody / 40S Ribosomal Protein S12 Antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human 40S Ribosomal Protein S12 (amino acids M1-S232) was used as the immunogen for the RPS12 Antibody.

Storage

After reconstitution, the RPS12 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

RPS12 antibody, 40S Ribosomal Protein S12 antibody, Ribosomal Protein S12 antibody, Small Ribosomal Subunit Protein S12 antibody, uS12 antibody, Ribosomal Protein S12e antibody

RPS12 Antibody / 40S Ribosomal Protein S12 Antibody (FY13479)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

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NSJ Bioreagents