RPLP0 Antibody / 60S acidic ribosomal protein P0 (RQ6798)

RPLP0 Antibody IF. Immunofluorescent staining of FFPE human A431 cells using RPLP0 Antibody / Ribosomal Protein P0 Antibody demonstrates strong cytoplasmic and perinuclear green fluorescence consistent with the intracellular localization of RPLP0. As a core component of the 60S ribosomal subunit and ribosomal stalk complex, RPLP0 plays an essential role in protein synthesis by facilitating interactions between the ribosome and translation factors required for efficient mRNA translation. The observed staining pattern is consistent with the widespread cytoplasmic distribution expected for a ribosomal protein involved in cellular protein production and translational regulation. Nuclei are counterstained with DAPI (blue). HIER was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Green = RPLP0 antibody; Blue = DAPI nuclear stain.

RPLP0 Antibody FACS. Flow cytometry analysis of human HL60 cells stained with RPLP0 Antibody / Ribosomal Protein P0 Antibody demonstrates a clear rightward shift of the antibody-stained population (blue) relative to the isotype control (green), consistent with positive detection of RPLP0 expression. RPLP0 is a highly conserved component of the 60S ribosomal subunit and serves as the central structural protein of the ribosomal stalk complex involved in protein synthesis and translational regulation. The observed fluorescence shift supports expression of RPLP0 in HL60 cells and is consistent with the ubiquitous cellular distribution expected for a ribosomal protein required for efficient mRNA translation and cellular protein production. Cells were stained with 1 ug antibody per million cells following blocking with goat serum. Red = cells alone; Green = isotype control; Blue = RPLP0 antibody.

RPLP0 Antibody WB. Western blot analysis of human 293T (lane 1), HeLa (lane 2), Jurkat (lane 3), and A375 (lane 4) cell lysates using RPLP0 Antibody / Ribosomal Protein P0 Antibody demonstrates strong immunoreactive bands at approximately 34 kDa in all samples tested. RPLP0 is a highly conserved component of the 60S ribosomal subunit and serves as the central structural protein of the ribosomal stalk complex, where it facilitates interactions between ribosomes and translation factors required for efficient protein synthesis. The consistent detection of RPLP0 across multiple human cell lines reflects its ubiquitous expression and essential role in cellular protein production, translational regulation, and maintenance of normal cellular function. Predicted molecular weight: approximately 34 kDa.