RPL3 Antibody | FY13562 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	FY13562	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

RPL3 Antibody by WB in human and rodent lysates. Western blot analysis using Large ribosomal subunit protein uL3 Antibody detects a band near 46 kDa in human Jurkat (lane 1), human Raji (lane 2), rat testis (lane 3), rat spleen (lane 4), mouse testis (lane 5), and mouse spleen (lane 6). The major band corresponds to the approximately 46 kDa expected mass of RPL3. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

RPL3 Antibody by WB in human and rodent cell and tissue lysates. Western blot analysis using Large ribosomal subunit protein uL3 Antibody detects a band near 46 kDa in human Jurkat (lane 1), human 293T (lane 2), rat kidney (lane 3), rat PC-12 (lane 4), mouse kidney (lane 5), and mouse RAW264.7 (lane 6). The major band corresponds to the approximately 46 kDa expected mass of RPL3. Reduced lysates containing 30 ug protein per lane were separated by 8% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 1:1000 overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

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RPL3 Antibody by WB in human and rodent lysates. Western blot analysis using Large ribosomal subunit protein uL3 Antibody detects a band near 46 kDa in human Jurkat (lane 1), human Raji (lane 2), rat testis (lane 3), rat spleen (lane 4), mouse testis (lane 5), and mouse spleen (lane 6). The major band corresponds to the approximately 46 kDa expected mass of RPL3. Reduced lysates containing 30 ug protein per lane were separated by 10% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

RPL3 Antibody by WB in human and rodent cell and tissue lysates. Western blot analysis using Large ribosomal subunit protein uL3 Antibody detects a band near 46 kDa in human Jurkat (lane 1), human 293T (lane 2), rat kidney (lane 3), rat PC-12 (lane 4), mouse kidney (lane 5), and mouse RAW264.7 (lane 6). The major band corresponds to the approximately 46 kDa expected mass of RPL3. Reduced lysates containing 30 ug protein per lane were separated by 8% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 1:1000 overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit Ig
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
UniProt	P39023
Applications	Western Blot : 0.5-1ug/ml ELISA : 0.1-0.5 ug/ml
Limitations	Intended solely for research purposes, this RPL3 Antibody / Large ribosomal subunit protein uL3 Antibody is not for clinical use.
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Description

RPL3 Antibody / Large ribosomal subunit protein uL3 Antibody detects RPL3, also designated uL3 under the universal ribosomal protein nomenclature. It is a component of the large 60S subunit of cytoplasmic ribosomes. Ribosomes translate messenger RNA into polypeptides, and their activity depends on the coordinated assembly of ribosomal RNA and numerous ribosomal proteins into structurally precise complexes.

uL3 is located near functionally important regions of the large ribosomal subunit and contributes to the architecture of the ribosome. The catalytic peptidyl-transferase activity of the ribosome is carried out by ribosomal RNA, but ribosomal proteins help stabilize the surrounding structure and support productive translation. RPL3 is therefore integral to the integrity of the translation machinery rather than a stand-alone peptide-bond-forming enzyme.

Ribosome production begins in the nucleolus, where precursor ribosomal RNAs are transcribed and processed while ribosomal proteins are assembled into developing subunits. Disturbances in ribosome biogenesis can influence cell growth and activate stress-response pathways. RPL3 has also been studied in relation to the regulation of cell-cycle and stress-associated programs, although proposed extraribosomal activities should be considered separately from its core structural role.

Protein synthesis is especially demanding in cells undergoing rapid growth or differentiation, making the coordination of ribosome assembly and translational capacity biologically important. RPL3 participates in this process as part of the 60S subunit and helps maintain the structure needed for efficient mRNA translation. NSJ Bioreagents provides the RPL3 Antibody for studies centered on ribosomal proteins and translation. An RPL3 Antibody can aid investigation of large-subunit organization and the cellular regulation of protein synthesis.

Explore related research targets in our Cell Biology Antibodies collection.

Application Notes

For best results, determine the optimal working dilution of the RPL3 Antibody / Large ribosomal subunit protein uL3 Antibody experimentally.

Immunogen

The RPL3 Antibody was generated using e. coli-derived human RPL3 recombinant protein (amino acids K28-K334).

Storage

Following reconstitution, the RPL3 Antibody can be kept at 4oC for one month. Aliquot for extended storage at -20oC for up to six months, avoiding repeated freezing and thawing.

Alternate Names

RPL3 antibody, Large ribosomal subunit protein uL3 antibody, ribosomal protein L3 antibody, 60S ribosomal protein L3 antibody, HIV-1 TAR RNA-binding protein B antibody, OK/SW-cl.32 antibody

RPL3 Antibody / Large ribosomal subunit protein uL3 Antibody (FY13562)

Description

Application Notes

Immunogen

Storage

Alternate Names

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents