RPL22 Antibody / Large ribosomal subunit protein eL22 Antibody (FY13565)

RPL22 Antibody by IF in HeLa cells. Immunofluorescence staining of HeLa cells using Large ribosomal subunit protein eL22 Antibody reveals punctate cytoplasmic green fluorescence with some perinuclear enrichment. Target immunoreactivity is shown in green, with alpha-tubulin in red and DAPI-labeled nuclei in blue. Cells were treated with enzyme antigen retrieval reagent, blocked with 10% goat serum and incubated with primary antibody at 5 ug/mL overnight at 4oC. Fluorescent secondary antibodies were used for microscopy.

RPL22 Antibody by WB in human and mouse cell lysates. Western blot analysis using Large ribosomal subunit protein eL22 Antibody detects a band near 15 kDa in human HepG2 (lane 1), HeLa (lane 2), Jurkat (lane 3), and mouse NIH/3T3 (lane 4). The dominant band corresponds to the approximately 15 kDa expected molecular weight of RPL22. Reduced lysates containing 30 ug protein per lane were separated by 12% SDS-PAGE and transferred to nitrocellulose. After blocking with 5% nonfat milk, membranes were incubated with primary antibody at 0.5 ug/mL overnight at 4oC, followed by HRP-conjugated secondary antibody and chemiluminescent detection.

RPL22 Antibody by IHC in mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded mouse brain using Large ribosomal subunit protein eL22 Antibody shows light brown staining in neuronal cell bodies and surrounding tissue. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum and incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen were used to visualize immunoreactivity, with blue nuclear counterstaining.

RPL22 Antibody by IHC in rat brain. Immunohistochemistry of formalin-fixed, paraffin-embedded rat brain using Large ribosomal subunit protein eL22 Antibody shows light brown immunoreactivity in neuronal cell bodies. Sections underwent heat-mediated antigen retrieval in EDTA buffer (pH 8.0), followed by blocking with 10% goat serum and incubation with primary antibody at 2 ug/mL overnight at 4oC. HRP-conjugated secondary antibody and DAB chromogen were used to visualize immunoreactivity, with blue nuclear counterstaining.