RPL18 Antibody | 60S ribosomal protein L18

	Catalog No	Formulation	Size	Price (USD)
	RQ8534	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	439
Formulation

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IHC staining of FFPE human placental tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human rectum adenocarcinoma tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver cancer tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human testicular germ cell tumor tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse colon tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat colon tissue with RPL18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human A549 cells with RPL18 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of 1) human HeLa, 2) human 293T, 3) human HepG2, 4) human Daudi, 5) rat testis, 6) rat brain, 7) mouse testis and 8) mouse brain tissue lysate with RPL18 antibody. Predicted molecular weight ~22 kDa.

Flow cytometry testing of fixed and permeabilized human HeLa cells with RPL18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPL18 antibody.

Availability	1-3 days
Species Reactivity	Human, Mouse, Rat
Format	Antigen affinity purified
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity purified
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	Q07020
Localization	Cytoplasm
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Immunofluorescence : 5ug/ml Flow Cytometry : 1-3ug/million cells ELISA : 0.1-0.5ug/ml
Limitations	This RPL18 antibody is available for research use only.
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Description

60S ribosomal protein L18 is a protein that in humans is encoded by the RPL18 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Application Notes

Optimal dilution of the RPL18 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids K19-A177) was used as the immunogen for the RPL18 antibody.

Storage

After reconstitution, the RPL18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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RPL18 Antibody / 60S ribosomal protein L18 / Large ribosomal subunit protein eL18 (RQ8534)

Description

Application Notes

Immunogen

Storage

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Area of Research

Product Type

Protocols

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