ROCK1 Antibody / Rho-associated protein kinase 1 (RQ7871)

Immunofluorescence analysis of ROCK1 expression in SiHa cells. Human SiHa cells were stained with anti-ROCK1 antibody (green) and anti-Alpha Tubulin antibody (red), followed by fluorophore-conjugated secondary antibodies. ROCK1 signal is observed prominently in the nuclear region with additional cytoplasmic staining, while Alpha Tubulin labels the cytoplasmic microtubule network. Although ROCK1 is classically described as a cytoplasmic kinase associated with actin and membrane-linked structures, nuclear localization has been reported in the literature under specific cellular contexts, consistent with the staining pattern observed here.

Immunohistochemistry analysis of ROCK1 expression in human breast cancer tissue. Paraffin-embedded human breast cancer tissue sections were subjected to heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and incubated with anti-ROCK1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen development. Brown staining is observed in tumor epithelial cells, consistent with cytoplasmic ROCK1 expression, with hematoxylin nuclear counterstain (blue).

Immunohistochemistry analysis of ROCK1 expression in mouse brain tissue. Paraffin-embedded mouse brain tissue sections were subjected to heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and incubated with anti-ROCK1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen development. ROCK1 immunoreactivity is detected in neural cells and surrounding neuropil, with hematoxylin nuclear counterstain (blue).

Immunohistochemistry analysis of ROCK1 expression in rat brain tissue. Paraffin-embedded rat brain tissue sections were subjected to heat-mediated antigen retrieval in EDTA buffer (pH 8.0) and incubated with anti-ROCK1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen development. Brown staining is observed in neuronal cells, consistent with cytoplasmic ROCK1 localization, with hematoxylin nuclear counterstain (blue).

Western blot analysis of ROCK1 expression. Whole cell lysates or tissue lysates from human HeLa cells (lane 1), human Jurkat cells (lane 2), rat brain tissue (lane 3), rat C6 cells (lane 4), mouse brain tissue (lane 5), and mouse NIH/3T3 cells (lane 6) were separated by SDS-PAGE and probed with anti-ROCK1 antibody. A specific immunoreactive band is detected at approximately 158 kDa across the samples, corresponding to ROCK1. This band is consistent with the predicted molecular weight of ROCK1 based on its amino acid sequence.