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Home >> Antibodies >> Ro60 Antibody / SS-A / TROVE2

Ro60 Antibody / SS-A / TROVE2 (RQ7591)

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  Catalog No Formulation Size Price (USD)  
Image RQ7591 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human Daudi cells with Ro60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ro60 antibody.
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Flow cytometry testing of human Daudi cells with Ro60 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ro60 antibody.
Western blot testing of 1) human MCF7, 2) human Jurkat, 3) human 293T, 4) human HeLa, 5) rat thymus and 6) rat spleen tissue lysate with Ro60 antibody. Predicted molecular weight ~61 kDa.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P10155
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ro60 antibody is available for research use only.
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Description

Enables RNA binding activity. Involved in cellular response to interferon-alpha and regulation of gene expression. Located in cytosol and nucleoplasm. Part of ribonucleoprotein complex. The cDNA that encodes the 60-kD Ro/SSA autoantigen recognized by autoantibodies in certain lupus erythematosus (152700) and Sjogren syndrome (270150) sera was cloned and sequenced by Deutscher et al. (1988) and confirmed with minor differences by Ben-Chetrit et al. (1989).

Application Notes

Optimal dilution of the Ro60 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids K88-M419) was used as the immunogen for the Ro60 antibody.

Storage

After reconstitution, the Ro60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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