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Home >> Antibodies >> Rnf8 Antibody

Rnf8 Antibody (RQ5760)

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  Catalog No Formulation Size Price (USD)  
Image RQ5760 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of mouse RAW264.7 cells with Rnf8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rnf8 antibody.
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Flow cytometry testing of mouse RAW264.7 cells with Rnf8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rnf8 antibody.
Western blot testing of 1) rat brain, 2) mouse brain and 3) NIH 3T3 lysate with Rnf8 antibody. Predicted molecular weight ~56 kDa but may be observed at larger sizes due to ubiquitination.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q8VC56
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Rnf8 antibody is available for research use only.
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Description

E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Rnf8 antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids E3-S488) was used as the immunogen for the Rnf8 antibody.

Storage

After reconstitution, the Rnf8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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