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Home >> Antibodies >> RNA Helicase A Antibody / DHX9

RNA Helicase A Antibody / DHX9 (RQ5970)

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  Catalog No Formulation Size Price (USD)  
Image RQ5970 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human gastric cancer with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
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Immunofluorescent staining of FFPE human gastric cancer with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with RNA Helicase A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse liver with RNA Helicase A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HEK293, 3) Jurkat, 4) K562, 5) Caco-2, 6) SW620, 7) Raji and 8) A549 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
Western blot testing of 1) rat PC-12, 2) mouse brain and 3) mouse RAW264.7 lysate with RNA Helicase A antibody. Predicted molecular weight ~140 kDa.
Flow cytometry testing of human HL60 cells with RNA Helicase A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNA Helicase A antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q08211
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This RNA Helicase A antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human
    Pred. Reactivity : Cow, Mouse, Rat

Description

ATP-dependent RNA helicase A (RHA; also known as DHX9, LKP, and NDHI) is an enzyme that in humans is encoded by the DHX9 gene. It is mapped to 1q25.3. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.

Application Notes

Optimal dilution of the RNA Helicase A antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E396-E759) was used as the immunogen for the RNA Helicase A antibody.

Storage

After reconstitution, the RNA Helicase A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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