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Home >> Antibodies >> RIG-I Antibody / DDX58

RIG-I Antibody / DDX58 (R32826)

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  Catalog No Formulation Size Price (USD)  
Image R32826 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus, 5) mouse NIH3T3 and 6) human Jurkat lysate with RIG-I antibody at 0.5ug/ml. Expected molecular weight: 106-115 kDa.
Western blot testing of 1) rat spleen, 2) rat thymus, 3) mouse spleen, 4) mouse thymus, 5) mouse NIH3T3 and 6) human Jurkat lysate with RIG-I antibody at 0.5ug/ml. Expected molecular weight: 106-115 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt O95786
Applications Western Blot : 0.5-1ug/ml
Limitations This RIG-I antibody is available for research use only.
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Description

RIG-I (retinoic acid-inducible gene I) is a RIG-I-like receptor dsRNA helicase enzyme that is encoded (in humans) by the DDX58 gene. RIG-I is part of the RIG-I-like receptor family, which also includes MDA5 and LGP2, and functions as a pattern recognition receptor that is a sensor for viruses such as influenza A, Sendai virus, and flavivirus. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.

Application Notes

Optimal dilution of the RIG-I antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids H871-K925 was used as the immunogen for the RIG-I antibody.

Storage

After reconstitution, the RIG-I antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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