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Home >> Antibodies >> Ribophorin II Antibody / RPN2

Ribophorin II Antibody / RPN2 (RQ7872)

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  Catalog No Formulation Size Price (USD)  
Image RQ7872 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-87 MG cells with Ribophorin II antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human U-87 MG cells with Ribophorin II antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) ThP-1, 2) HepG2 and 3) T-47D cell lysate with Ribophorin II antibody. Predicted molecular weight ~69 kDa.
Flow cytometry testing of human HL60 cells with Ribophorin II antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribophorin II antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P04844
Localization Cytoplasm (ER)
Applications Western blot : 0.5-1ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ribophorin II antibody is available for research use only.
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Description

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 2, also called Ribophorin II, is an enzyme that in humans is encoded by the RPN2 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the Ribophorin II antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Q64-Q174) was used as the immunogen for the Ribophorin II antibody.

Storage

After reconstitution, the Ribophorin II antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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