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Home >> Antibodies >> Ribonuclease Inhibitor Antibody / RNH1

Ribonuclease Inhibitor Antibody / RNH1 (R31956)

  Catalog No Formulation Size Price (USD)  
Image R31956 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat testis, 3) rat lung, 4) mouse brain, 5) mouse testis, 6) mouse lung and 7) mouse HEPA1-6 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
Western blot testing of 1) human placenta, 2) human HeLa, 3) human SK-O-V3, 4) human Jurkat, 5) human U-87 MG, 6) monkey COS-7, 7) human SW620 and 8) human Caco-2 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
Flow cytometry testing of human A431 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat, Monkey
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P13489
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Ribonuclease Inhibitor antibody is available for research use only.
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Description

Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.

Application Notes

Optimal dilution of the Ribonuclease Inhibitor antibody should be determined by the researcher.

Immunogen

Amino acids SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDD of human RNH1 were used as the immunogen for the Ribonuclease Inhibitor antibody.

Storage

After reconstitution, the Ribonuclease Inhibitor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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