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Home >> Antibodies >> Ribonuclease 3 Antibody / RNASE3 / Eosinophil cationic protein

Ribonuclease 3 Antibody / RNASE3 / Eosinophil cationic protein (RQ4467)

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  Catalog No Formulation Size Price (USD)  
Image RQ4467 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human Jurkat, 2) rat liver and 3) mouse liver lysate with Ribonuclease 3 antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
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Western blot testing of 1) human Jurkat, 2) rat liver and 3) mouse liver lysate with Ribonuclease 3 antibody at 0.5ug/ml. Predicted molecular weight ~18 kDa.
IHC testing of FFPE human ovarian cancer with Ribonuclease 3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human sarcoma with Ribonuclease 3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse small intestine with Ribonuclease 3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P12724
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml (human recombinant protein)
Limitations This Ribonuclease 3 antibody is available for research use only.
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Description

Eosinophil Cationic Protein (ECP) also known as Ribonuclease 3 is a basic protein located in the eosinophil primary matrix. In humans, the eosinophil cationic protein is encoded by the RNASE3 gene. The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. ECP is released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body.

Application Notes

Optimal dilution of the Ribonuclease 3 antibody should be determined by the researcher.

Immunogen

Human recombinant protein (amino acids R28-I160) was used as the immunogen for the Ribonuclease 3 antibody.

Storage

After reconstitution, the Ribonuclease 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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