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Home >> Antibodies >> RHOBTB2 Antibody / DBC2

RHOBTB2 Antibody / DBC2 [clone DBC2/3361] (V9500)

  Catalog No Formulation Size Price (USD)  
Image V9500-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9500-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9500SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human renal oncocytoma with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid tissue with RHOBTB2 antibody (clone DBC2/3361) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free RHOBTB2 antibody (clone DBC2/3361) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using RHOBTB2 antibody (clone DBC2/3361). These results demonstrate the foremost specificity of the DBC2/3361 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name DBC2/3361
Purity Protein A/G affinity
UniProt Q9BYZ6
Localization Secreted in plasma
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This RHOBTB2 antibody is available for research use only.
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Description

The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. DBC-2 (deleted in breast cancer 2 gene protein), also known as RHOBTB2 (Rho-related BTB domain-containing protein 2), is a 727 amino acid member of the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. Expressed ubiquitously with highest levels in neural tissue, heart, brain and fetal lung, DBC-2 contains two BTB (POZ) domains through which it may bind to and regulate the function of target proteins, such as CUL-3. Additionally, DBC-2 is thought to function as a regulator of cell cycle and apoptosis events. Under normal conditions, DBC-2 is thought to exhibit tumor suppressor activity. Mutations in the gene encoding DBC-2 are associated with breast cancer, suggesting that mutated DBC-2 may play a role in carcinogenesis.

Application Notes

Optimal dilution of the RHOBTB2 antibody should be determined by the researcher.

Immunogen

A portin of amino acids 554-604 from the human protein was used as the immunogen for the RHOBTB2 antibody.

Storage

Aliquot the RHOBTB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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