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Home >> Antibodies >> RGS14 Antibody

RGS14 Antibody (R31283)

  Catalog No Formulation Size Price (USD)  
Image R31283 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of RGS14 antibody and Lane 1: rat thymus; 2: (r) spleen; 3: human Raji; 4: (h) CEM; 5: (h) Jurkat cell lysate. Expected/observed size ~65KD
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt O43566
Applications Western blot : 0.5-1ug/ml
Limitations This RGS14 antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human

Description

Regulator of G-protein signaling 14 is a protein that in humans is encoded by the RGS14 gene. This gene encodes a member of the regulator of G-protein signaling family. By genomic sequence analysis, this gene is mapped to chromosome 5q35.3. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein(GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the RGS14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the C-terminus of human Regulator of G-protein signaling 14 (CTVLADQEVRLENR) was used as the immunogen for this RGS14 antibody (100% homologous in human, mouse and rat).

Storage

After reconstitution, the RGS14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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