RENT1 Antibody / hUPF1 [clone 11E7.] (RQ5658)

Immunohistochemistry analysis of RENT1 / hUPF1 antibody in human breast cancer tissue. FFPE human breast carcinoma shows cytoplasmic HRP-DAB brown staining in tumor epithelial cells, consistent with RENT1 expression. Staining is predominantly cytoplasmic with variable intensity among malignant cells, while nuclei are counterstained blue. Adjacent stromal elements demonstrate comparatively weaker staining. Heat induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8.0, for 20 minutes followed by cooling prior to immunostaining.

IHC staining of FFPE mouse intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE rat intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of FFPE human U-2 OS cells with RENT1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Western blot testing of human 1) HepG2, 2) Raji, 3) PC-3, 4) HeLa and 5) HEK293 lysate with RENT1 antibody. Predicted molecular weight ~124 kDa, but routinely observed at 130-140 kDa.

Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse RAW264.7 lysate with RENT1 antibody. Predicted molecular weight ~124 kDa, but routinely observed at 130-140 kDa.