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Home >> Antibodies >> Renal Lineage Marker PAX8 Antibody | PAX8 Protein

Renal Lineage Marker PAX8 Antibody | PAX8 Protein [clone MSVA-708R] (V6133)

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  Catalog No Formulation Size Price (USD)  
Image V6133-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 654.50
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V6133-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
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Immunohistochemistry analysis of PAX8 antibody in human tissue microarrays. Formalin-fixed, paraffin-embedded normal and cancer tissue microarrays were stained with Paired box protein Pax-8 rabbit recombinant monoclonal antibody (clone MSVA-708R). Nuclear HRP-DAB brown staining is observed in expected PAX8-positive tissues, including renal tubular epithelium, thyroid follicular cells, and Mullerian-derived epithelium, while most non-lineage tissues show minimal to no nuclear signal. In cancer tissues, strong nuclear staining is present in renal cell carcinoma, thyroid carcinoma, and Mullerian-derived tumors, consistent with known lineage-specific expression patterns. The staining distribution across normal and malignant samples aligns with reported expression profiles in the Human Protein Atlas. Negative tissues demonstrate absence of specific nuclear staining, supporting specificity of the antibody.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-708R
UniProt Q06710
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1:100-1:200
Limitations This Renal Lineage Marker PAX8/PAX8 Protein antibody is available for research use only.
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Description

Renal Lineage Marker PAX8 antibody recognizes PAX8 protein, a nuclear transcription factor encoded by the PAX8 gene and a critical regulator of renal epithelial differentiation. PAX8 protein is a member of the paired box family of transcription factors and plays a central role in kidney development and epithelial lineage specification. Because of its consistent expression in renal tubular epithelial cells, PAX8 antibody is widely used in research focused on renal biology and renal-derived tumors.

PAX8 contains a conserved paired DNA-binding domain that enables regulation of genes involved in organogenesis and epithelial differentiation. During embryonic kidney development, PAX8 contributes to nephric lineage commitment and maturation of renal epithelial structures. In adult tissues, PAX8 protein remains expressed in renal tubular epithelial cells, where it maintains aspects of epithelial identity. Detection of PAX8 protein therefore reflects renal epithelial lineage and differentiation status.

PAX8 protein localizes to the nucleus, consistent with its role as a transcription factor. Beyond the kidney, PAX8 expression is also observed in thyroid follicular cells and Mullerian-derived epithelium, including fallopian tube and endometrium. In pathologic settings, PAX8 expression is commonly retained in renal cell carcinomas, including clear cell, papillary, and chromophobe subtypes. Because of this expression pattern, PAX8 antibody is frequently used in studies evaluating tumor origin and distinguishing renal carcinomas from other malignancies.

The PAX8 gene is located on chromosome 2q14 and produces multiple isoforms through alternative splicing. Dysregulated expression and gene rearrangements involving PAX8 have been reported in thyroid and other epithelial tumors. Renal Lineage Marker PAX8 antibody clone MSVA-708R is a recombinant rabbit monoclonal antibody developed to detect PAX8 protein in research applications. As a recombinant monoclonal antibody, clone MSVA-708R supports consistent nuclear detection of PAX8 protein in renal epithelial tissues and related tumor models.

Application Notes

1. Optimal dilution of the Renal Lineage Marker PAX8/PAX8 Protein antibody should be determined by the researcher.

2. This Renal Lineage Marker PAX8/PAX8 Protein antibody is recombinantly produced by expression in human HEK293 cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 150-300) of human PAX8 (exact sequence is proprietary) was used as the immunogen for the Renal Lineage Marker PAX8/PAX8 Protein antibody.

Storage

Renal Lineage Marker PAX8/PAX8 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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