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Home >> Antibodies >> Recombinant YWHAE Antibody / 14-3-3E

Recombinant YWHAE Antibody / 14-3-3E [clone rYWHAE/8860] (V4462)

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  Catalog No Formulation Size Price (USD)  
Image V4462-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4462-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4462SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human prostate carcinoma tissue with recombinant YWHAE antibody (clone rYWHAE/8860). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with recombinant YWHAE antibody (clone rYWHAE/8860). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Rat
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rYWHAE/8860
Purity Protein A/G affinity
UniProt P62258
Localization Cytoplasm, Melanosome
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant YWHAE antibody is available for research use only.
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Description

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. The YWHAE gene which encodes 14-3-3E has also been identified as a possible susceptibility gene for schizophrenia.

Application Notes

Optimal dilution of the recombinant YWHAE antibody should be determined by the researcher.

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant YWHAE antibody.

Storage

Aliquot the recombinant YWHAE antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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