vWF Antibody Clone VWF/1859R / von Willebrand Factor Antibody [clone VWF/1859R] (V3491)

vWF Antibody Clone VWF/1859R immunohistochemistry analysis. IHC staining of FFPE human tonsil using vWF Antibody Clone VWF/1859R (clone VWF/1859R) demonstrates endothelial-specific HRP-DAB brown staining within vascular structures, highlighting blood vessel linings amid surrounding lymphoid tissue. The staining pattern is consistent with von Willebrand factor (VWF) localization to endothelial cells, with signal confined to vessel-associated cells and absent in adjacent lymphocytes. Antigen retrieval was performed using heat-induced epitope retrieval (HIER) in pH 9 Tris-EDTA buffer, supporting robust visualization of vascular endothelium in tonsillar tissue.

vWF Antibody Clone VWF/1859R western blot analysis. Western blot of 1) partial recombinant protein and 2) human lung lysate using vWF Antibody Clone VWF/1859R (clone VWF/1859R). Lane 1 shows a lower molecular weight band corresponding to a truncated recombinant fragment, while lane 2 shows a prominent band at approximately 250 kDa, consistent with the predicted molecular weight of von Willebrand factor (VWF). The higher molecular weight signal in lung lysate reflects the heavily glycosylated nature of VWF, which undergoes extensive post-translational modification and multimerization, often resulting in an elevated or broadened apparent molecular weight on SDS-PAGE relative to the core protein.

SDS-PAGE analysis of purified, BSA-free recombinant vWF antibody (clone VWF/1859R) as confirmation of integrity and purity.