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Home >> Antibodies >> Recombinant von Willebrand Factor Antibody

Recombinant von Willebrand Factor Antibody [clone rVWF/1465] (V3686)

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  Catalog No Formulation Size Price (USD)  
Image V3686-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3686-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3686SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V3686IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
Microvalidated Recmousemono
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IHC testing of FFPE human tonsil with recombinant von Willebrand Factor antibody (clone rVWF/1465). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil with recombinant von Willebrand Factor antibody (clone rVWF/1465). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of 1) partial recombinant protein and 2) human lung lysate with recombinant von Willebrand Factor antibody (clone rVWF/1465). Predicted molecular weight ~250 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant von Willebrand Factor antibody (clone rVWF/1465) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rVWF/1465
Purity Protein G affinity chromatography
UniProt P04275
Localization Cytoplasmic
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This recombinant von Willebrand Factor antibody is available for research use only.
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Description

von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Application Notes

The optimal dilution of the recombinant von Willebrand Factor antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Amino acids 1815-1939 from the human protein were used as the immunogen for this recombinant von Willebrand Factor antibody.

Storage

Store the recombinant von Willebrand Factor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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