Vimentin Antibody / Cellular Plasticity Marker Antibody [clone VIM/6576R] (V9766)

Vimentin Antibody / Cellular Plasticity Marker Antibody. Immunohistochemistry analysis of FFPE human tonsil tissue using Vimentin antibody shows strong HRP-DAB brown cytoplasmic staining in stromal and mesenchymal cell populations, with minimal staining in most lymphoid cells. The rabbit monoclonal clone VIM/6576R reveals heterogeneous staining intensity and filament organization across the tissue, with regions of dense, interconnected networks adjacent to areas of lighter, more diffuse cytoplasmic signal. This variability in staining pattern is consistent with differences in cellular state and supports the use of Vimentin as a marker of cellular plasticity within stromal compartments.

IHC staining of FFPE human tonsil tissue with recombinant Vimentin antibody (clone VIM/6576R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Vimentin Antibody / Cellular Plasticity Marker Antibody for WB. Western blot analysis of human U-87 MG cell lysate using Vimentin antibody detects a prominent band at approximately 55 kDa, consistent with the predicted molecular weight of Vimentin (VIM). The rabbit monoclonal clone VIM/6576R shows a clear band, supporting detection of intermediate filament components associated with adaptable cellular states. The presence of Vimentin in U-87 MG cells aligns with its role in cytoskeletal organization linked to phenotypic flexibility and cellular plasticity.

SDS-PAGE analysis of purified, BSA-free recombinant Vimentin antibody (clone VIM/6576R) as confirmation of integrity and purity.